Skip to main content

Posts

Showing posts from June, 2020

Advances in hemoglobin A1c point of care technology.

Measurement of hemoglobin A1c (A1C) has long been accepted as the best indicator of glucose control over time. A1C tests for the technology used by both differences in costs (high pressure liquid chromatography) or structure (boronate affinity or immunoassay combined with general chemistry). This technology is commonly used in expensive laboratory instruments. More recently, technology A1C has been put into place care (POC) devices, allowing for the availability  Simian Clia Kits of measurement of A1C, greatly facilitate diabetes care in both specialist and general practice.  A1C POC tests must have acceptable performance, standardization of national reference, the National Glycohemoglobin Standardization Program (NGSP) certification, simple operation without the need for expensive instrumentation, and the Clinical Laboratory Improvement Amendments (CLIA) waiver. CLIA-waived POC technologies including Bio-Rad MicroMat II (distributed by a Cholestech GDX) and Axis-Shield Afi...

Validation of laboratory-developed molecular assays for infectious diseases.

molecular technology has transformed the way that clinical laboratories to diagnose and manage many infectious diseases. excellent sensitivity, specificity, and speed has made molecular tests an attractive alternative to a culture or enzyme immunoassay methods. Many molecular tests that are commercially available and FDA approved. Others, particularly those tests for analytes  Bovine Clia Kits less common, often laboratory developed. Laboratory tests are also often modify FDA approved for inserting different extraction systems or other types of additional specimens.  The Clinical Laboratory Improvement Amendments (CLIA) federal regulatory standards that require clinical laboratories to establish and document their own performance specifications for laboratory tests developed to ensure accurate results and just before the execution of the test. The performance characteristics to be formed include accuracy, precision, various reported, hose reference, analytical sensitivity an...

Assessment of Etest as an alternative to agar dilution for antimicrobial susceptibility testing of Neisseria gonorrhoeae.

We studied whether the Etest can be used as an alternative to dilution in order to determine the antimicrobial susceptibility of ceftriaxone, cefixime and cefpodoxime in the supervision of Neisseria gonorrhoeae. One hundred fifteen clinical and laboratory  Caprine Clia Kits isolates of N. gonorrhoeae were tested following the Clinical Laboratory Improvement Amendments (CLIA) approved CLSI standard dilution methods to ensure and, separately, with Etest according to the manufacturer's recommendations. The MIC is determined and compared.  The resulting mutant laboratory used to simulate ten specimens substantially nonsusceptible. The Etest and agar dilution method were well correlated. The resulting statistical test regression R2 values ​​of 88%, 82%, and 85% and the Pearson correlation coefficient of 92%, 91%, and 92% for ceftriaxone, cefixime and cefpodoxime, respectively. When pairwise comparisons were made, two tests were 88.7%, 80%, and 87% within 1 log2 dilution of each oth...

Infrastructure for Personalized Medicine at Partners HealthCare.

Partners HealthCare Personalized Medicine (PPM) is central in partner systems HealthCare (founded by Massachusetts General Hospital and Brigham and Women's Hospital) whose mission is to utilize genetics and genomics to improve patient care in a cost effective manner.  PPM consists of five interrelated  Equine Clia Kits components: (1) Laboratory of Molecular Medicine (LMM), CLIA laboratories perform genetic testing for patients around the world; (2) Translational Genomics Core (TGC), a core lab provides a platform for researchers Partners genome; (3) Options Biobank, a Biobank samples (DNA, plasma and serum) for 50,000 patients agree Partners; (4) Biobank Portal, IT infrastructure and the viewer to unify genotype, sample, phenotype (validated diagnosis, radiology, and clinical chemistry) of electronic medical records researchers Partners. These components are held together by (5) common IT system that brings researchers, clinicians, and patients together for optimal research...

Incidence of the 22q11.2 deletion in a large cohort of miscarriage samples.

BACKGROUND The 22q11.2 deletion syndrome is the most common microdeletion syndrome in live births, but data on the incidence in other populations is limited and also includes ascertainment bias. This study was designed to determine the incidence of miscarriage deletion 22q11.2  Human Clia Kits in samples sent for testing clinical molecular cytogenetics. RESULTS 6101 Twenty fresh products of conception (POC) samples were sent to CLIA- certified, CAP-accredited laboratory of April 2010 - May 2016 for miscarriage molecular cytogenetic testing using single nucleotide polymorphism (SNP) based microarray platform. A retrospective review determined 22q11.2 deletion events in this sample set. The results obtained in 22,451 fetuses (86%) cases, which, 15 (0.07%) had a microdeletion at 22q11.2 region (incidence, 1/1497). Of those, 12 (80%) cases were found in a sample of normal at a resolution karyotyping traditional (ie, no chromosomal abnormalities in over 10 Mb in size) and three cases (2...

Sero-epidemiological study in prediction of the risk groups for measles outbreaks in Vojvodina, Serbia.

Serological survey provides insight grouped age gap measles-specific immunity and the expected prevalence of a certain age. The purpose  Ovine Clia Kits of this study was to describe the sero-epidemiology of measles in Vojvodina before the outbreak in 2017/18 and to discuss preventive measures for the future potential epidemics. A seroprevalence study was conducted from April 2015 to June 2017 by the 3199 serum samples leftovers , The study was conducted prior to the last measles outbreak in Vojvodina which occurred between 12 November 2017 and June 30, 2018. Measles-specific IgG antibodies were determined by using indirect chemiluminescent immunoassay (CLIA) registered participants .Median age was 20 years (IQR 11- 37). Overall, 86.9% of serum samples were seropositive. The highest proportion of measles seronegativity observed in children aged 12-23 months and in adults aged 20-39 years (56.1% and 18.5%, respectively).  The prevalence of measles seronegativity above the WHO...